CO-UTILIZATION OF GLUCOSE AND XYLOSE INCREASES GROWTH RATE WITHOUT AFFECTING PLASMID DNA YIELD OF ENGINEERED E. coli

 

R.M. Gálvez, T.E. Pablos, J.C. Sigala and A.R. Lara

 

 

  • Glucose is a better carbon source than xylose for pDNA and biomass synthesis by E. coli PTS- GalP+ Δ (recA deoR).

  • The simultaneous consumption of glucose and xylose by E. coli PTS- GalP+ Δ (recA deoR) increased growth rate without a significant effect on pDNA yield.

  • The use on glucose-xylose mixtures is an easy and effective strategy to improve pDNA production by engineered E. coli strains.